My personal projects on how to apply life science in the home for food preservation, food enhancement, and alcohol production.
Microorganisms were actually only first identified in the late 1600s by dutch scientist Anton van Leeuwenhoek, commonly known as the father of microbiology. However, humans have been utilising them since before recorded history to make alcohol, bread, or to keep food safe for longer periods. Some people even speculate that the production of bread and alcohol was what drove humans to settle and found civilisation in the first place!
Life science and microbiology then does not need to be confined to the lab and can be explored at home. Different species or even strains of the same species can have remarkably different effects on the food in question. The main aim is to cultivate an environment that favours the growth or beneficial species through salting, drying, or high sugar concentrations. There is a lot of freedom to experiment with flavours and truly delicious things can be made with just a little understanding of our natural world.
It is important to note that while home fermentation and preservation are largely safe, there are obviously inherent risks. Risks are specific to each type of fermentation. In general however, make sure to research the processes thoroughly from multiple sources before attempting to do them yourself and maintain the cleanliness and sterility of all equipment used. Never attempt to consume food that smells off, has swollen its packaging, produces large amounts of unexpected gas, or otherwise seems spoiled. If you are unsure what constitutes spoiled, there is an age old adage to follow. If in doubt, chuck it out!
Primary microorganism used: Saccharomyces cerevisiae (Lalvin 71B brewer's strain)
Historically called the nectar of the gods, mead is an alcoholic drink that used honey as the primary fermentable sugar. This mead used 1.6 kg of honey in a final volume of 4.5 L. Flavourings such as fruit or spices can be added, and 5 g of a mono cultured wine yeast strain (Lalvin 71B in this instance) is then added to begin fermentation. A hydrometer is used to measure alcohol production, relying on the different specific gravity between sugar and ethanol. Alternatively, the alcohol content can be calculated from the available sugars and the alcohol tolerance of the yeast. Though wild yeasts can be used for this process, they are by nature much less consistent with unknown flavour profiles or alcohol tolerances.
Juniper berry and orange mead during the initial fermentation, observe how it has become cloudy
Raspberry and cinnamon mead during the initial fermentation, note the bubbles, this ferment was active
Airlock (allows gas release and prevents high pressures and explosions)
After the primary fermentation the yeast falls to the bottom as the alcohol content is now too high for survival, clearing the mead
The mead is then siphoned off to remove the yeast and flavourings (siphoning is important to reduce oxygenation)
Finally, the mead is bottled, sealed with a cork and allowed to bottle age for 3-12 months to let the flavours improve
Primary microorganisms used: species of the Lactobacilli genus
A Korean national dish, kim chi is fermented napa cabbage. Traditionally seasoned with gochujang and gochugaru (Korean fermented chilli paste and chili powder). Wild lactic acid bacteria that live on the surface of the cabbage are selected for by the generation of a salty environment which the comparatively halo-tolerant Lactobacilli can survive. This is achieved by weighing the ingredients and the addition of 2% (w/v) salt to the final weight. The salt draws water from the cabbage, creating a brine that . Fermentation occurs at room temperature for 4-7 days before transfer to the fridge to slow the process down. Though under proper condition the kim chi will not really go bad, the longer it ferments the more sour and soft the cabbage will become. Therefore, the duration of the fermentation is simply a matter of taste.
Gochujang (fermented Korean chili paste)
Kim chi before fermentation
Kim chi after fermentation (and consumption)
Primary microorganisms used: species of the Lactobacilli genus
A fantastic addition to any sandwich. Most pickles bought in shops are vinegar pickled. Lacto-fermentaion provides a unique flavour and acidity that can't be found otherwise. Safe and effective fermentation is nurtured, as with kim chi, through the establishment of a salty environment that favours halophilic lactic acid bacteria. Unlike Kim chi though, in this instance this is achieved by submerging the pickles in a 3% (w/v) salt solution. Fermentation can occur at room temperature for a week before the process is slowed by refrigeration. Like the kim chi, the longer the fermentation the more sour and soft the pickles become.
Before fermentation cucumbers (clear brine)
After fermentation finished pickles (cloudy brine)
Cross section of fermented pickle
Primary microorganisms used: General lactic acid bacteria of the order Lactobacillales (commonly Staphylococcus xylosus)
Curing meat can also be performed with the assistance of bacteria. As duck breast is small and safe to eat cured it makes a good candidate for home attempts. Two essential considerations for this are a high salinity and a low moisture. This is achieved by packing the breast in salt for several days which draws out excess moisture and allows the salt to penetrate the meat. Excess salt is then removed and the breast is then wrapped and allowed to dry further and age in a cool environment. Beneficial bacteria then grow, curing the meat and producing a flavour similar to parma ham. As the salinity in this process is comparatively much higher than for kim chi. The growth of cultures is much slower, and curing can take weeks to months of proper aging.
Initial salting of the duck breast
Topside of the aged duck breast
Underside of the aged duck breast
Slicing the finished product
Primary microorganisms used: SCOBY (symbiotic culture of bacteria and yeast) comprised primarily of Saccharomyces cerevisiae yeast (wild strains) and bacterial species of the Lactobacilli genus
The SCOBY is the starter culture used to leaven the bread in the production of sourdough. A SCOBY is easily grown at home by mixing an even ratio of flour and water and leaving it at room temperature. Wild yeasts and bacteria present in the flour then begin to grow. Every day half of the substrate is removed and replaced with fresh medium. This is actually similar to how a chemostat operates! After a week, the SCOBY should be bubbling and active enough to begin the process of bread making. As long as you feed the culture daily it can persist almost indefinitely. For longer storage, the culture can also be refrigerated or frozen.
The mother (SCOBY)
Shaping and decoration
The loaf after baking
Cross section of the loaf
Primary microorganisms used: Acetic acid bacteria of the family Acetobacteraceae
Though not strictly a biological process, chutneys nonetheless use biological principles for food preservation and enhancement. The combination of vinegar and sugar make an acidic and hydro gyroscopic environment that is inimicable to microbial life. Vinegar can be easily made yourself through the addition of unpasteurised vinegar to any alcoholic drink. The acetic acid bacteria then convert the ethanol to acetic acid in what is called a secondary or double fermentation. Though vinegar is cheap and widely available, this is a great way to make dressings with specific flavours, or to use up homemade alcohols that didn't turn out super palatable.
Mango chutney
Mustard piccalilli
St. John's chutney
Tomato and chili chutney